Mouse GR beta (glucocorticoid receptor beta) CLIA Kit
The Mouse GR beta (Glucocorticoid Receptor beta) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of GR beta levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for various research applications.The glucocorticoid receptor beta plays a crucial role in regulating the body's response to stress and inflammation. Dysregulation of this receptor has been linked to various diseases, including metabolic disorders, immune conditions, and neurological disorders.
Therefore, the accurate measurement of GR beta levels is essential for understanding its role in these conditions and developing potential therapeutic interventions.Overall, the Mouse GR beta CLIA Kit offers researchers a valuable tool for studying the function of glucocorticoid receptor beta in mouse models, ultimately advancing our understanding of its role in health and disease.
Product Name:
Mouse GR beta (glucocorticoid receptor beta) CLIA Kit
SKU:
MOES00292
Target:
Mouse GR beta (glucocorticoid receptor beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GRβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GRβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GRβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GRβ. You can calculate the concentration of Mouse GRβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
90-102
87-99
Average (%)
95
95
93
1:4
Range (%)
87-103
88-101
102-117
Average (%)
94
95
108
1:8
Range (%)
96-108
99-112
100-112
Average (%)
103
106
106
1:16
Range (%)
88-101
99-113
96-107
Average (%)
95
106
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.63
72.22
207.92
26.6
75.34
209.68
Standard deviation
2.24
7.06
14.45
2.27
7.02
17.28
C V (%)
8.11
9.78
6.95
8.53
9.32
8.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GRβ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GRβ in samples. No significant cross-reactivity or interference between Mouse GRβ and analogues was observed.
