The Mouse GRN (Granulin) CLIA Kit is specifically designed for the precise measurement of granulin levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing accurate and consistent results that are essential for various research applications.Granulin is a key protein that plays a vital role in cell growth, inflammation, and tissue repair. It is implicated in various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.
By utilizing the Mouse GRN CLIA Kit, researchers can gain valuable insights into the role of granulin in disease development and progression, ultimately advancing our understanding of the mechanisms underlying these conditions and paving the way for innovative treatment strategies.
Product Name:
Mouse GRN (Granulin) CLIA Kit
SKU:
MOES00309
Target:
Mouse GRN (Granulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GRN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GRN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GRN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GRN. You can calculate the concentration of Mouse GRN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-116
90-106
90-104
Average (%)
106
97
96
1:4
Range (%)
99-113
90-104
94-107
Average (%)
107
96
101
1:8
Range (%)
99-113
84-96
85-97
Average (%)
107
90
90
1:16
Range (%)
86-99
102-116
95-108
Average (%)
92
110
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-111
105
EDTA plasma (n=5)
101-115
108
Cell culture media (n=5)
100-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
3.05
9.09
1.11
2.77
8.72
Standard deviation
0.13
0.27
0.71
0.12
0.24
0.57
C V (%)
11.93
8.85
7.81
10.81
8.66
6.54
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GRN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GRN in samples. No significant cross-reactivity or interference between Mouse GRN and analogues was observed.
