Mouse Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g) ELISA Kit
The Mouse Growth Arrest and DNA Damage Inducible Protein Gamma (GADD45G) ELISA Kit is designed for the precise measurement of GADD45G levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.GADD45G is a key protein that plays a critical role in the response to DNA damage and cellular stress.
It is involved in regulating cell growth, apoptosis, and DNA repair processes, making it a valuable biomarker for studying diseases such as cancer, genetic disorders, and neurodegenerative conditions. The Mouse GADD45G ELISA Kit provides researchers with a reliable tool for investigating the function and expression of GADD45G, enabling advancements in understanding disease mechanisms and potential therapeutic targets.
Product Name:
Mouse Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g) ELISA Kit
Product Code:
MODL00516
Size:
96 Assays
Target:
GADD45g
Synonyms:
CR6, DDIT2, GADD45gamma, GRP17, Gadd-Related Protein,17 kD, Cytokine-responsive protein CR6, DNA damage-inducible transcript 2 protein
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-100
95
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
