The Mouse GS-Gelsolin CLIA Kit is a highly reliable and sensitive assay designed for the quantitative detection of GS-Gelsolin levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity and accuracy, ensuring consistent and reproducible results for a variety of research applications.GS-Gelsolin is a key protein involved in actin filament dynamics and cytoskeleton organization, playing a critical role in cell motility, migration, and cellular signaling pathways. Changes in GS-Gelsolin levels have been linked to various diseases and conditions, including cancer, inflammatory disorders, and neurodegenerative diseases, making it a valuable biomarker for understanding disease mechanisms and potential therapeutic interventions.
With its advanced technology and user-friendly design, the Mouse GS-Gelsolin CLIA Kit is an essential tool for researchers studying the role of GS-Gelsolin in health and disease. Get accurate and reliable results with this cutting-edge assay kit from Assay Genie.
Product Name:
Mouse GS (Gelsolin) CLIA Kit
SKU:
MOES00287
Target:
Mouse GS (Gelsolin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GS. You can calculate the concentration of Mouse GS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-117
87-98
87-100
Average (%)
109
93
92
1:4
Range (%)
86-99
99-114
86-100
Average (%)
91
105
91
1:8
Range (%)
90-103
99-111
100-116
Average (%)
95
105
108
1:16
Range (%)
90-104
103-118
91-102
Average (%)
95
109
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-108
102
EDTA plasma (n=5)
95-107
100
Cell culture media (n=5)
89-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.49
13.03
49.38
5.96
12.96
52.58
Standard deviation
0.65
1.52
4.67
0.59
1.54
5.41
C V (%)
11.84
11.67
9.46
9.9
11.88
10.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GS in samples. No significant cross-reactivity or interference between Mouse GS and analogues was observed.
