The Mouse GZMs-A (Granzyme A) CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of Granzyme A levels in mouse serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it perfect for a variety of research applications.Granzyme A is an important protein involved in immune responses, specifically in cytotoxic T lymphocytes and natural killer cells. It plays a critical role in cell-mediated cytotoxicity and apoptosis, making it a valuable biomarker for studying immune responses and inflammatory processes.
With its high sensitivity and specificity, the Mouse GZMs-A CLIA Kit is an essential tool for researchers looking to study immune responses, inflammation, and potential therapeutic targets related to Granzyme A. Get reliable and precise results with this advanced assay kit from Assay Genie.
Product Name:
Mouse Gzms-A (granzyme A) CLIA Kit
SKU:
MOES00311
Target:
Mouse Gzms-A (granzyme A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GzmA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GzmA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GzmA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GzmA. You can calculate the concentration of Mouse GzmA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
85-96
91-104
Average (%)
100
90
97
1:4
Range (%)
99-112
98-115
96-108
Average (%)
104
105
101
1:8
Range (%)
87-101
94-108
89-103
Average (%)
92
101
96
1:16
Range (%)
100-116
100-113
94-111
Average (%)
106
106
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
104
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
96-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.99
219.05
896.56
91.93
201.33
938.64
Standard deviation
9.33
22.83
79.79
9.12
22.91
102.97
C V (%)
9.62
10.42
8.9
9.92
11.38
10.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GzmA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GzmA in samples. No significant cross-reactivity or interference between Mouse GzmA and analogues was observed.
