The Mouse GZMS-B (Granzyme B) ELISA Kit is specifically designed for the precise measurement of granzyme B levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Granzyme B is an important enzyme that plays a crucial role in the immune response by inducing apoptosis in target cells, especially in the context of cytotoxic T lymphocytes and natural killer cells. Its dysregulation has been implicated in various diseases, including autoimmune disorders and viral infections, making it a valuable biomarker for investigating these conditions and exploring potential therapeutic interventions.
The Mouse GZMS-B ELISA Kit from Assay Genie provides researchers with a reliable tool for studying the functions of granzyme B in immune responses and disease pathogenesis. Its easy-to-use format and comprehensive instructions make it a convenient choice for studies aimed at unraveling the complexities of immune-mediated diseases and developing targeted treatments.
Product Name:
Mouse Gzms-B (granzyme B) ELISA Kit
SKU:
MOES01103
Target:
Mouse Gzms-B (granzyme B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GzmB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GzmB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GzmB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GzmB. You can calculate the concentration of Mouse GzmB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
93-105
95-110
Average (%)
100
98
103
1:4
Range (%)
91-108
82-94
83-93
Average (%)
99
88
88
1:8
Range (%)
94-109
82-92
84-96
Average (%)
99
87
89
1:16
Range (%)
89-101
83-96
86-101
Average (%)
95
88
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
91
EDTA plasma (n=5)
94-108
100
Cell culture media (n=5)
93-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.92
155.11
363.59
51.09
158.5
378.21
Standard deviation
3.56
9.1
16.33
3.43
7.04
16.79
C V (%)
6.99
5.87
4.49
6.71
4.44
4.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse GzmB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GzmB in samples. No significant cross-reactivity or interference between Mouse GzmB and analogues was observed.
