The Mouse HB-EGF (Heparin-Binding Epidermal Growth Factor-Like Growth Factor) CLIA Kit is a cutting-edge tool for researchers looking to accurately measure HB-EGF levels in mouse serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing precise and consistent results for various experimental applications.HB-EGF is a key factor in cellular growth and development, particularly in processes such as wound healing and tissue regeneration. Its role in promoting cell proliferation and migration makes it a vital component in studies related to cancer, cardiac diseases, and skin disorders.
By accurately measuring HB-EGF levels, researchers can gain valuable insights into these conditions and potentially uncover new therapeutic approaches.With the Mouse HB-EGF CLIA Kit from AssayGenie, researchers can confidently explore the intricate workings of HB-EGF and its implications in various physiological and pathological processes. Elevate your research capabilities with this innovative kit designed to deliver accurate and reliable results.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HB-EGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HB-EGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HB-EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HB-EGF. You can calculate the concentration of Mouse HB-EGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
91-107
93-105
Average (%)
100
98
100
1:4
Range (%)
94-110
99-118
95-107
Average (%)
102
107
101
1:8
Range (%)
91-106
99-118
94-105
Average (%)
98
107
100
1:16
Range (%)
97-113
98-113
93-106
Average (%)
103
103
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-114
108
EDTA plasma (n=5)
100-115
106
Cell culture media (n=5)
94-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.55
43.56
186.52
26.51
46.06
197.33
Standard deviation
2.56
3.86
20.42
2.9
4.09
20.76
C V (%)
9.64
8.86
10.95
10.94
8.88
10.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HB-EGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HB-EGF in samples. No significant cross-reactivity or interference between Mouse HB-EGF and analogues was observed.
