The Mouse Hepc (Hepcidin) CLIA Kit is specifically designed for the precise quantification of hepcidin levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Hepcidin is a key regulator of iron homeostasis, controlling iron absorption and distribution in the body. Dysregulation of hepcidin levels can lead to disorders such as anemia, hemochromatosis, and other iron-related conditions.
Therefore, accurate measurement of hepcidin is crucial for understanding these diseases and developing effective treatments.This Mouse Hepc (Hepcidin) CLIA Kit is a valuable tool for researchers studying iron metabolism, anemia, and other related disorders in mouse models. Its reliable performance and ease of use make it an essential component for any laboratory conducting research in this field.
Product Name:
Mouse Hepc (Hepcidin) CLIA Kit
SKU:
MOES00365
Target:
Mouse Hepc (Hepcidin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Hepc. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Hepc and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Hepc, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Hepc. You can calculate the concentration of Mouse Hepc in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
92-108
97-114
Average (%)
96
99
104
1:4
Range (%)
103-117
97-112
88-100
Average (%)
109
104
95
1:8
Range (%)
90-104
89-100
90-102
Average (%)
96
94
96
1:16
Range (%)
92-104
90-103
93-106
Average (%)
97
95
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
95
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
83-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.04
302.39
988.5
100.07
298.43
904.19
Standard deviation
10.27
26.19
79.48
10.59
34.32
81.2
C V (%)
9.33
8.66
8.04
10.58
11.5
8.98
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Hepc concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Hepc in samples. No significant cross-reactivity or interference between Mouse Hepc and analogues was observed.
