Description
Mouse HGF (Hepatocyte Growth Factor) ELISA Kit
The Mouse Hepatocyte Growth Factor (HGF) ELISA Kit is expertly designed for the precise quantification of HGF levels in a variety of biological samples. HGF, a potent growth factor with diverse biological functions, plays a pivotal role in cellular proliferation, migration, and regeneration, particularly in the context of liver regeneration, kidney development, and wound healing processes. This ELISA kit offers exceptional sensitivity and specificity, enabling accurate measurement of HGF levels and providing valuable insights into its involvement in various physiological and pathological processes.
Manufactured under rigorous quality control standards, this kit ensures robust performance, reproducibility, and ease of use, making it an ideal choice for cutting-edge research and biomedical investigations.
| Product Name: | Mouse HGF (Hepatocyte Growth Factor) ELISA Kit |
| Product Code: | AEES00228 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 31.25-2000 pg/mL |
| Sensitivity: | 18.75 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Mouse HGF in samples. No significant cross-reactivity or interference between Mouse HGF and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse HGF concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HGF. You can calculate the concentration of Mouse HGF in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
