The Mouse HHIP (Hedgehog Interacting Protein) CLIA Kit is specifically designed for the accurate quantification of HHIP levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results that are perfect for a variety of research applications.HHIP is a key protein that interacts with the hedgehog signaling pathway, impacting cell growth and differentiation. Dysregulation of this pathway has been implicated in various diseases, including cancer and developmental disorders.
Therefore, measuring HHIP levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its exceptional performance and ease of use, the Mouse HHIP CLIA Kit is an essential tool for researchers studying hedgehog signaling and its implications in disease progression. Order now and take your research to the next level.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HHIP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HHIP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HHIP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HHIP. You can calculate the concentration of Mouse HHIP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
94-109
88-99
Average (%)
93
101
93
1:4
Range (%)
98-111
90-105
91-106
Average (%)
104
97
99
1:8
Range (%)
90-103
93-108
90-105
Average (%)
96
99
98
1:16
Range (%)
84-100
95-108
101-115
Average (%)
91
102
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-100
91
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
89-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.57
306.95
761.35
93.3
307.5
787.51
Standard deviation
11.98
32.57
68.45
10.48
28.23
64.42
C V (%)
12.54
10.61
8.99
11.23
9.18
8.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HHIP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HHIP in samples. No significant cross-reactivity or interference between Mouse HHIP and analogues was observed.
