The Mouse HNF4A (Hepatocyte Nuclear Factor 4 Alpha) CLIA Kit is a highly reliable and accurate assay designed for the quantitative detection of HNF4A levels in mouse serum, plasma, and cell lysates. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.HNF4A is a critical transcription factor involved in the regulation of genes related to liver development, glucose metabolism, and lipid homeostasis. Dysregulation of HNF4A has been implicated in various metabolic disorders, including diabetes and fatty liver disease.
As such, measuring HNF4A levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.With its user-friendly protocol and high performance characteristics, the Mouse HNF4A CLIA Kit is an indispensable tool for researchers studying liver biology, metabolic diseases, and related pathways. Trust AssayGenie's expertise to help advance your research efforts and uncover new insights into the role of HNF4A in health and disease.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HNF4a. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HNF4a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HNF4a, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HNF4a. You can calculate the concentration of Mouse HNF4a in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
94-110
103-118
Average (%)
100
101
109
1:4
Range (%)
96-112
95-110
93-107
Average (%)
104
102
101
1:8
Range (%)
85-97
94-109
96-113
Average (%)
92
100
104
1:16
Range (%)
95-107
93-106
92-108
Average (%)
101
99
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
102
EDTA plasma (n=5)
97-110
105
Cell culture media (n=5)
100-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.01
137.97
417.26
51.55
129.23
442.7
Standard deviation
5.42
16.35
33.09
6.4
14.63
48.12
C V (%)
11.06
11.85
7.93
12.42
11.32
10.87
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HNF4a concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HNF4a in samples. No significant cross-reactivity or interference between Mouse HNF4a and analogues was observed.
