The Mouse HNF6B (Hepatocyte Nuclear Factor 6 Beta) CLIA Kit is a powerful tool for accurate measurement of HNF6B levels in mouse samples. This kit offers exceptional sensitivity and specificity, guaranteeing precise and consistent results for a variety of research purposes.HNF6B is a critical transcription factor that plays a key role in liver development and function, as well as in regulating glucose and lipid metabolism. Dysregulation of HNF6B has been implicated in liver diseases such as hepatitis and cirrhosis, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its innovative design and reliable performance, the Mouse HNF6B CLIA Kit is an indispensable solution for researchers seeking to delve deeper into the molecular mechanisms governing liver biology and disease progression.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HNF6b. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HNF6b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HNF6b, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HNF6b. You can calculate the concentration of Mouse HNF6b in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
94-112
91-104
Average (%)
102
102
98
1:4
Range (%)
86-96
86-102
99-115
Average (%)
91
93
105
1:8
Range (%)
88-99
86-99
94-106
Average (%)
93
92
101
1:16
Range (%)
102-115
91-105
90-106
Average (%)
108
96
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
103
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
92-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.47
48.3
191.11
24.32
46.75
173.02
Standard deviation
2.83
4.52
14.94
3.05
3.75
13.03
C V (%)
11.57
9.36
7.82
12.54
8.02
7.53
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HNF6b concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HNF6b in samples. No significant cross-reactivity or interference between Mouse HNF6b and analogues was observed.
