The Mouse HO-1 (Heme Oxygenase-1) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of HO-1 levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications in the study of oxidative stress, inflammation, and cellular protection mechanisms.HO-1 is a critical enzyme involved in the breakdown of heme into carbon monoxide, biliverdin, and iron, playing a key role in antioxidant defense and cellular homeostasis. Dysregulation of HO-1 has been linked to various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions, making it a valuable biomarker for assessing disease progression and potential therapeutic interventions.
With its high sensitivity and specificity, the Mouse HO-1 CLIA Kit offers researchers a powerful tool for studying the role of HO-1 in health and disease, providing valuable insights into the mechanisms underlying oxidative stress and inflammation. This kit is essential for advancing our understanding of HO-1 biology and potential therapeutic strategies for a range of pathological conditions.
Product Name:
Mouse HO-1 (Heme Oxygenase 1) CLIA Kit
SKU:
MOES00351
Target:
Mouse HO-1 (Heme Oxygenase 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HO1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HO1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HO1. You can calculate the concentration of Mouse HO1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
90-102
86-97
Average (%)
92
96
91
1:4
Range (%)
91-104
95-113
96-111
Average (%)
97
103
102
1:8
Range (%)
96-109
87-102
85-96
Average (%)
102
94
91
1:16
Range (%)
94-108
88-102
95-110
Average (%)
100
95
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
93-105
100
Cell culture media (n=5)
98-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.04
65.83
199.19
23.91
60.73
192.19
Standard deviation
3.25
4.85
17.03
3.04
4.78
16.05
C V (%)
12.98
7.37
8.55
12.71
7.87
8.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HO1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HO1 in samples. No significant cross-reactivity or interference between Mouse HO1 and analogues was observed.
