The Mouse HP (Haptoglobin) CLIA Kit is specifically designed for the precise measurement of haptoglobin levels in mouse serum samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an invaluable tool for various research studies.Haptoglobin is a crucial protein involved in the immune response, binding to free hemoglobin to prevent oxidative damage and inflammation.
Elevated haptoglobin levels are associated with various inflammatory conditions, making it a key biomarker for monitoring and studying these diseases.With its user-friendly protocol and reliable performance, the Mouse HP (Haptoglobin) CLIA Kit is a valuable asset for researchers investigating inflammatory responses and immune system function in mice models.
Product Name:
Mouse HP (Haptoglobin) CLIA Kit
SKU:
MOES00325
Target:
Mouse HP (Haptoglobin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HP. You can calculate the concentration of Mouse HP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
95-106
94-109
Average (%)
109
101
100
1:4
Range (%)
94-108
88-100
100-116
Average (%)
99
95
107
1:8
Range (%)
97-111
95-110
89-105
Average (%)
102
100
96
1:16
Range (%)
94-104
92-108
99-112
Average (%)
99
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
90-102
95
Cell culture media (n=5)
98-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.56
156.33
472.54
47.06
168.86
461.74
Standard deviation
5.22
16.1
35.02
5.76
13.34
47.14
C V (%)
10.98
10.3
7.41
12.24
7.9
10.21
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HP in samples. No significant cross-reactivity or interference between Mouse HP and analogues was observed.
