The Mouse HSP-60 (Heat Shock Protein 60) CLIA Kit is a highly sensitive and specific assay designed for the detection of HSP-60 levels in mouse serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it suitable for a variety of research applications.HSP-60 is a vital chaperone protein involved in maintaining cellular homeostasis and protecting cells from stress-induced damage. Dysregulation of HSP-60 has been implicated in various diseases including cancer, inflammation, and neurodegenerative disorders, highlighting its importance as a potential diagnostic and therapeutic target.
With its superior performance and ease of use, the Mouse HSP-60 CLIA Kit is an essential tool for researchers studying the role of HSP-60 in health and disease, providing valuable insights into its molecular mechanisms and potential clinical applications.
Product Name:
Mouse HSP-60 (Heat Shock Protein 60) CLIA Kit
SKU:
MOES00327
Target:
Mouse HSP-60 (Heat Shock Protein 60)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Hsp-60. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Hsp-60 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Hsp-60, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Hsp-60. You can calculate the concentration of Mouse Hsp-60 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
100-118
88-103
Average (%)
100
108
95
1:4
Range (%)
86-101
86-102
94-110
Average (%)
92
93
101
1:8
Range (%)
92-107
93-107
83-95
Average (%)
99
100
90
1:16
Range (%)
84-99
104-120
102-118
Average (%)
90
110
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
93-107
100
Cell culture media (n=5)
94-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.22
241.58
939.43
96.72
264.77
974.56
Standard deviation
12.27
21.98
57.59
12.4
31.35
78.26
C V (%)
12.37
9.1
6.13
12.82
11.84
8.03
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Hsp-60 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Hsp-60 in samples. No significant cross-reactivity or interference between Mouse Hsp-60 and analogues was observed.
