SynonymsMTPB,Mitochondrial Trifunctional Protein,Beta Subunit,3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A Hydratase(Trifunctional Protein)Beta Subunit,Beta-ketothiolase
ReactivityMouse
Sample TypeTissue Homogenates And Other Biological Fluids
SKU:
MODL00604
UPC:
Product Type:
ELISA Kit
Sensitivity:
0.115ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
MTPB,Mitochondrial Trifunctional Protein,Beta Subunit,3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A Hydratase(Trifunctional Protein)Beta Subunit,Beta-ketothiolase
Mouse Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) ELISA Kit
The Mouse Hydroxyacyl-Coenzyme A Dehydrogenase β (HADHB) ELISA Kit is a reliable tool for the quantitative detection of HADHB levels in mouse serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring accurate and reproducible results that are ideal for a variety of research applications.HADHB is a key enzyme involved in fatty acid metabolism and beta-oxidation, playing a crucial role in energy production and lipid metabolism.
Dysregulation of HADHB has been associated with various metabolic disorders, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.With the Mouse Hydroxyacyl-Coenzyme A Dehydrogenase β (HADHB) ELISA Kit, researchers can confidently analyze HADHB levels in mouse samples, advancing their understanding of metabolic pathways and metabolic disorders.
Product Name:
Mouse Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) ELISA Kit
Product Code:
MODL00604
Size:
96 Assays
Target:
HADHb
Synonyms:
MTPB, Mitochondrial Trifunctional Protein,Beta Subunit, 3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A Hydratase(Trifunctional Protein)Beta Subunit, Beta-ketothiolase
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
