The Mouse Intact Parathormone (PTH) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of intact PTH levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Intact PTH is a key hormone involved in calcium regulation and bone metabolism. Dysregulation of PTH levels can lead to various bone disorders, such as osteoporosis and hyperparathyroidism.
Monitoring intact PTH levels is crucial for studying these conditions and potential therapeutic interventions.With its high sensitivity and specificity, the Mouse Intact PTH CLIA Kit from Assaygenie is a valuable tool for researchers studying bone health and calcium metabolism in mice.
Product Name:
Mouse I-PTH (intact Parathormone) CLIA Kit
SKU:
MOES00393
Target:
Mouse I-PTH (intact Parathormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse I-PTH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse I-PTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse I-PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse I-PTH. You can calculate the concentration of Mouse I-PTH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
91-105
99-112
Average (%)
104
97
105
1:4
Range (%)
85-101
95-108
85-99
Average (%)
92
100
92
1:8
Range (%)
100-119
102-113
91-103
Average (%)
108
107
97
1:16
Range (%)
96-108
89-103
95-111
Average (%)
103
94
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
96
EDTA plasma (n=5)
91-102
96
Cell culture media (n=5)
100-115
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.95
41.85
188.44
24.78
42.34
169.9
Standard deviation
2.45
3.4
16.43
2.22
4.48
13.49
C V (%)
10.23
8.12
8.72
8.96
10.58
7.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse I-PTH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse I-PTH in samples. No significant cross-reactivity or interference between Mouse I-PTH and analogues was observed.
