The Mouse IAPP (Islet Amyloid Polypeptide) ELISA Kit is a high-quality assay kit designed for the accurate quantification of IAPP levels in mouse serum, plasma, and cell culture supernatants. This kit features a high level of sensitivity and specificity, ensuring precise and reliable results for a wide range of research applications.IAPP is a key peptide hormone that plays a crucial role in regulating glucose homeostasis and energy metabolism.
Dysregulation of IAPP is implicated in the pathogenesis of type 2 diabetes, making it a valuable biomarker for studying diabetes and related metabolic disorders. This ELISA kit provides researchers with a powerful tool for investigating the role of IAPP in disease pathogenesis and developing potential therapeutic interventions.
Product Name:
Mouse IAPP (Islet Amyloid Polypeptide) ELISA Kit
SKU:
MOES01735
Target:
Mouse IAPP (Islet Amyloid Polypeptide)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with IAPP. During the reaction, IAPP in the sample or standard competes with a fixed amount of IAPP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to IAPP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IAPP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
97-111
86-102
Average (%)
101
103
93
1:4
Range (%)
90-102
94-110
97-113
Average (%)
97
100
105
1:8
Range (%)
84-98
88-100
93-106
Average (%)
90
94
99
1:16
Range (%)
84-96
89-102
97-112
Average (%)
91
96
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
85-97
90
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
206.7
359.7
1896.0
215.7
355.1
1856.6
Standard deviation
14.1
15.8
96.7
10.8
20.6
102.1
C V (%)
6.82
4.39
5.1
5.01
5.8
5.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of IAPP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes IAPP in samples. No significant cross-reactivity or interference between IAPP and analogues was observed.
