I apologize for any confusion, but the URL you provided leads to a Mouse ICAM-1 (Intercellular Adhesion Molecule-1) CLIA Kit, not a Human ANG (Angiogenin) ELISA Kit. Therefore, I will provide a product description based on the information from the URL:The Mouse ICAM-1 CLIA Kit is specifically designed for the accurate measurement of ICAM-1 levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.
ICAM-1 is a critical cell surface glycoprotein involved in mediating cell-to-cell adhesion, particularly in inflammatory and immune responses. Its expression is upregulated in various diseases such as atherosclerosis, autoimmune disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Mouse ICAM-1 CLIA Kit offers researchers a powerful tool for investigating the role of ICAM-1 in disease pathogenesis and developing new treatment strategies.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ICAM-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ICAM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ICAM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ICAM-1. You can calculate the concentration of Mouse ICAM-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-118
86-98
95-108
Average (%)
107
91
101
1:4
Range (%)
103-118
99-111
90-104
Average (%)
109
104
95
1:8
Range (%)
99-111
99-114
86-98
Average (%)
106
106
91
1:16
Range (%)
89-103
98-112
89-101
Average (%)
95
106
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
99
EDTA plasma (n=5)
95-108
103
Cell culture media (n=5)
92-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
191.99
578.3
1886.02
207.53
546.87
1712.8
Standard deviation
16.28
42.39
146.54
24.76
57.31
105.51
C V (%)
8.48
7.33
7.77
11.93
10.48
6.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ICAM-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ICAM-1 in samples. No significant cross-reactivity or interference between Mouse ICAM-1 and analogues was observed.
