The Mouse IgE (Immunoglobulin E) CLIA Kit is specifically designed for the quantitative measurement of IgE levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and precision, providing accurate and reproducible results for a variety of research applications.Immunoglobulin E is an important antibody involved in allergic responses, triggering the release of inflammatory mediators in response to allergens. Elevated IgE levels are associated with conditions such as asthma, eczema, and allergic rhinitis, making this kit an invaluable tool for studying allergic diseases and developing targeted therapies.
With its easy-to-use protocol and reliable performance, the Mouse IgE CLIA Kit from Assay Genie is a valuable addition to any research laboratory focused on immunology, allergy, and inflammation. Order now to advance your research and uncover new insights into the role of IgE in allergic diseases.
Product Name:
Mouse IgE (Immunoglobulin E) CLIA Kit
SKU:
MOES00376
Target:
Mouse IgE (Immunoglobulin E)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IgE. You can calculate the concentration of Mouse IgE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
91-105
92-106
Average (%)
101
96
98
1:4
Range (%)
99-113
86-97
96-111
Average (%)
107
91
103
1:8
Range (%)
93-107
92-107
97-113
Average (%)
99
98
105
1:16
Range (%)
102-120
99-114
96-110
Average (%)
110
107
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
92-106
100
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.51
4.82
0.55
1.64
5.23
Standard deviation
0.06
0.16
0.31
0.07
0.12
0.45
C V (%)
11.32
10.6
6.43
12.73
7.32
8.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IgE concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IgE in samples. No significant cross-reactivity or interference between Mouse IgE and analogues was observed.
