The Mouse Immunoglobulin E (IgE) ELISA Kit is meticulously developed for the precise quantitative analysis of IgE levels in diverse biological samples, aiming to uncover insights into immune responses and allergic reactions. Immunoglobulin E is a crucial antibody involved in the activation of inflammatory responses and allergic conditions by triggering immune cells, particularly mast cells and basophils.
This ELISA kit boasts exceptional sensitivity and specificity, enabling accurate measurement of IgE levels and enhancing the understanding of its role in immune system regulation and allergic manifestations. Manufactured under stringent quality control measures, the kit guarantees reliable and reproducible results, making it an optimal choice for cutting-edge research and immunological studies.
Product Name:
Mouse IgE (Immunoglobulin E) ELISA Kit
SKU:
AEES00229
Target:
Mouse IgE (Immunoglobulin E)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
75.00 ng/mL
Detection range:
125.00-8000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgE. You can calculate the concentration of Mouse IgE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
88-98
98-108
Average (%)
94
93
104
1:4
Range (%)
87-95
87-97
99-106
Average (%)
91
91
102
1:8
Range (%)
102-113
86-94
96-106
Average (%)
105
91
103
1:16
Range (%)
100-110
99-109
84-99
Average (%)
104
105
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-111
104
EDTA plasma (n=5)
86-97
93
Cell culture media (n=5)
94-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
382.47
858.8
3319.11
351.27
946.2
3588.32
Standard deviation
21.34
40.54
153.34
19.81
46.65
191.98
C V (%)
5.58
4.72
4.62
5.64
4.93
5.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IgE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IgE in samples. No significant cross-reactivity or interference between Mouse IgE and analogues was observed.
