The Mouse IGF-1 (Insulin-like Growth Factor 1) ELISA Kit is a highly sensitive assay designed for precise quantification of IGF-1 levels in various biological samples from mouse models.
IGF-1 is a critical growth factor that plays pivotal roles in cellular proliferation, differentiation, and regulation of metabolic processes. It acts as a key mediator in promoting growth and development, tissue repair, and overall metabolic homeostasis. When using Assay Genie's Mouse IGF-1 ELISA Kit, researchers can expect exceptional sensitivity and specificity, ensuring accurate and reproducible results in assessing IGF-1 levels. With stringent quality control measures in place during manufacturing, this ELISA kit ensures robust performance and reliable data for research applications. Trust in Assay Genie's Mouse IGF-1 ELISA Kit for precise quantification of IGF-1 levels in your studies, providing valuable insights into growth regulation, metabolic activities, and related biological processes.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGF-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IGF-1. You can calculate the concentration of Mouse IGF-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
92-101
97-107
Average (%)
98
95
102
1:4
Range (%)
87-100
101-109
90-103
Average (%)
95
104
96
1:8
Range (%)
87-95
90-98
97-110
Average (%)
91
94
104
1:16
Range (%)
93-100
91-105
89-95
Average (%)
97
97
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-97
92
EDTA plasma (n=5)
89-100
97
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.54
117.97
459.78
45.45
123.54
450.44
Standard deviation
2.23
5.39
21.79
2.5
5.6
22.03
C V (%)
5.37
4.57
4.74
5.5
4.53
4.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IGF-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IGF-1 in samples. No significant cross-reactivity or interference between Mouse IGF-1 and analogues was observed.
