Mouse IGFBP-1 (Insulin-Like Growth Factor Binding Protein 1) CLIA Kit
The Mouse IGFBP-1 (Insulin-like Growth Factor Binding Protein 1) CLIA Kit is a powerful tool for the quantitative measurement of IGFBP-1 levels in mouse serum, plasma, and tissue homogenates. With excellent sensitivity and specificity, this kit ensures accurate and reliable results for a variety of research applications.IGFBP-1 is an important protein that plays a critical role in regulating the activity of insulin-like growth factors and modulating cell growth and differentiation.
Dysregulation of IGFBP-1 has been linked to various diseases, including diabetes, cancer, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and identifying potential therapeutic targets.Overall, the Mouse IGFBP-1 CLIA Kit is a valuable tool for researchers looking to explore the role of IGFBP-1 in health and disease, providing precise measurements for in-depth analysis and interpretation of experimental results.
Product Name:
Mouse IGFBP-1 (Insulin-Like Growth Factor Binding Protein 1) CLIA Kit
SKU:
MOES00389
Target:
Mouse IGFBP-1 (Insulin-Like Growth Factor Binding Protein 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGFBP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGFBP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IGFBP-1. You can calculate the concentration of Mouse IGFBP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
90-104
90-105
Average (%)
102
96
97
1:4
Range (%)
96-112
90-105
97-112
Average (%)
103
98
104
1:8
Range (%)
93-108
99-111
100-113
Average (%)
100
106
105
1:16
Range (%)
96-109
103-120
96-110
Average (%)
103
109
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
95
EDTA plasma (n=5)
91-102
96
Cell culture media (n=5)
86-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.77
199.09
789.05
110.25
207.3
867.39
Standard deviation
11.61
20.69
50.1
10.73
24.75
97.58
C V (%)
11.08
10.39
6.35
9.73
11.94
11.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IGFBP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IGFBP-1 in samples. No significant cross-reactivity or interference between Mouse IGFBP-1 and analogues was observed.
