Mouse IGFBP-6 (Insulin-Like Growth Factor Binding Protein 6) CLIA Kit
The Mouse IGFBP-6 (Insulin-Like Growth Factor Binding Protein 6) CLIA Kit is a highly reliable tool for measuring the levels of IGFBP-6 in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides accurate and reproducible results, making it an ideal choice for a variety of research applications.IGFBP-6 is a critical protein that binds to insulin-like growth factors, regulating their bioavailability and activity.
It plays a crucial role in cell growth, differentiation, and apoptosis, making it essential for understanding various physiological processes and disease pathways.Whether studying cancer, growth disorders, or other diseases related to insulin-like growth factors, the Mouse IGFBP-6 CLIA Kit is a valuable tool for researchers looking to investigate the role of IGFBP-6 and its implications in various biological processes.
Product Name:
Mouse IGFBP-6 (Insulin-Like Growth Factor Binding Protein 6) CLIA Kit
SKU:
MOES00392
Target:
Mouse IGFBP-6 (Insulin-Like Growth Factor Binding Protein 6)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP-6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGFBP-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGFBP-6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IGFBP-6. You can calculate the concentration of Mouse IGFBP-6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
97-112
87-101
Average (%)
102
103
93
1:4
Range (%)
89-103
99-112
89-102
Average (%)
97
106
95
1:8
Range (%)
84-96
98-111
101-116
Average (%)
90
104
108
1:16
Range (%)
85-97
99-113
90-105
Average (%)
91
107
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
106
EDTA plasma (n=5)
99-118
107
Cell culture media (n=5)
99-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.48
100.19
383.79
46.69
98.89
351.62
Standard deviation
6.03
8.64
25.1
5.0
7.94
27.36
C V (%)
12.97
8.62
6.54
10.71
8.03
7.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IGFBP-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IGFBP-6 in samples. No significant cross-reactivity or interference between Mouse IGFBP-6 and analogues was observed.
