Mouse IGFBP-6 (Insulin-like Growth Factor Binding Protein 6) ELISA Kit
The Mouse IGFBP-6 ELISA Kit is meticulously crafted for the precise quantitative assessment of Insulin-like Growth Factor Binding Protein 6 (IGFBP-6) levels in diverse biological samples. IGFBP-6 is a vital protein that plays a crucial role in modulating the actions of insulin-like growth factors, especially IGF-II, by binding to them and regulating their activity. This intricate interaction influences cell growth, differentiation, and survival, implicating IGFBP-6 in fundamental cellular processes. Accurate measurement of IGFBP-6 levels is essential for unraveling its intricate role in modulating IGF signaling pathways and cellular responses. Understanding the dynamics of IGFBP-6 can provide valuable insights into its impact on cell growth regulation, metabolic processes, and potential implications in various physiological and pathological conditions.
The Mouse IGFBP-6 ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring dependable and reproducible results. Crafted under strict quality control measures, this kit delivers robust performance and ease of use, making it a reliable tool for research investigations aimed at elucidating the functional significance of Insulin-like Growth Factor Binding Protein 6 in cellular physiology and disease pathophysiology.
Product Name:
Mouse IGFBP-6 (Insulin-like Growth Factor Binding Protein 6) ELISA Kit
SKU:
AEES00256
Target:
Mouse IGFBP-6 (Insulin-like Growth Factor Binding Protein 6)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGFBP-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGFBP-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IGFBP-6. You can calculate the concentration of Mouse IGFBP-6 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
87-100
88-101
Average (%)
93
94
95
1:4
Range (%)
87-99
87-98
93-106
Average (%)
92
93
98
1:8
Range (%)
83-96
88-100
89-104
Average (%)
89
95
95
1:16
Range (%)
90-102
92-104
84-98
Average (%)
95
97
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
101
EDTA plasma (n=5)
94-108
100
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.57
50.36
243.9
25.46
49.57
266.77
Standard deviation
1.7
2.21
12.41
1.42
2.57
14.06
C V (%)
6.63
4.39
5.09
5.56
5.19
5.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IGFBP-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IGFBP-6 in samples. No significant cross-reactivity or interference between Mouse IGFBP-6 and analogues was observed.
