The Mouse IL-10 CLIA Kit is specifically designed for the precise measurement of interleukin-10 (IL-10) levels in mouse serum, plasma, and tissue culture supernatants. This kit offers excellent sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.IL-10 is a key immunoregulatory cytokine that plays a crucial role in modulating immune responses and inflammation. It is involved in regulating the balance between pro-inflammatory and anti-inflammatory processes, making it a valuable biomarker for studying immune-related diseases such as autoimmune disorders, infectious diseases, and inflammatory conditions.
With its reliable performance and user-friendly format, the Mouse IL-10 CLIA Kit is an essential tool for researchers looking to investigate the role of IL-10 in immune function and disease pathogenesis. Order now to advance your research and further explore the complexities of the immune system.
Product Name:
Mouse IL-10 CLIA Kit
SKU:
MOES00043
Target:
Mouse IL-10
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-10. You can calculate the concentration of Mouse IL-10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
84-97
99-115
Average (%)
96
91
107
1:4
Range (%)
94-107
101-116
98-112
Average (%)
99
107
104
1:8
Range (%)
99-112
101-118
100-116
Average (%)
106
108
109
1:16
Range (%)
96-108
91-104
98-111
Average (%)
101
97
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
91
EDTA plasma (n=5)
89-99
94
Cell culture media (n=5)
100-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.2
70.96
194.11
28.97
71.52
189.94
Standard deviation
2.57
5.06
20.25
3.14
8.17
20.51
C V (%)
9.45
7.13
10.43
10.84
11.42
10.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-10 in samples. No significant cross-reactivity or interference between Mouse IL-10 and analogues was observed.
