The Mouse IL-11 CLIA Kit is a powerful tool for the precise measurement of interleukin-11 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.Interleukin-11 is a key cytokine involved in regulating various biological processes, including inflammation, hematopoiesis, and bone metabolism.
It plays a crucial role in diseases such as inflammatory bowel disease, osteoporosis, and cancer, making it a valuable biomarker for studying these conditions and exploring potential treatments.With the Mouse IL-11 CLIA Kit, researchers can uncover new insights into the role of interleukin-11 in health and disease, advancing our understanding and paving the way for innovative therapeutic interventions.
Product Name:
Mouse IL-11 CLIA Kit
SKU:
MOES00406
Target:
Mouse IL-11
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-11. You can calculate the concentration of Mouse IL-11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
93-106
100-113
Average (%)
107
101
105
1:4
Range (%)
88-101
97-110
86-100
Average (%)
95
103
93
1:8
Range (%)
102-117
91-105
103-119
Average (%)
108
97
110
1:16
Range (%)
101-113
90-102
94-107
Average (%)
108
95
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-113
103
EDTA plasma (n=5)
85-99
91
Cell culture media (n=5)
93-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.93
42.33
242.59
24.7
40.28
251.28
Standard deviation
2.55
4.42
18.41
2.64
3.52
27.09
C V (%)
9.47
10.44
7.59
10.69
8.74
10.78
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-11 in samples. No significant cross-reactivity or interference between Mouse IL-11 and analogues was observed.
