The Mouse IL-12 CLIA Kit is a reliable assay designed for the quantitative detection of IL-12 levels in mouse serum, plasma, and tissue homogenates. This kit boasts high sensitivity and specificity, providing accurate and reproducible results for a variety of research applications.IL-12 is a key cytokine involved in the regulation of immune responses, particularly in the development of T cells and natural killer cells. Dysregulation of IL-12 has been implicated in autoimmune disorders, infectious diseases, and inflammatory conditions, highlighting its significance as a potential biomarker for studying these diseases and evaluating therapeutic interventions.
With the Mouse IL-12 CLIA Kit, researchers can confidently measure IL-12 levels in mouse samples, paving the way for further insights into the role of this cytokine in immune system function and disease pathology.
Product Name:
Mouse IL-12 CLIA Kit
SKU:
MOES00407
Target:
Mouse IL-12
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-12. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-12, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-12. You can calculate the concentration of Mouse IL-12 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
88-100
96-113
Average (%)
93
94
103
1:4
Range (%)
97-108
92-105
100-116
Average (%)
102
97
106
1:8
Range (%)
103-117
91-105
89-104
Average (%)
109
96
96
1:16
Range (%)
90-102
101-116
91-103
Average (%)
95
108
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-115
106
EDTA plasma (n=5)
83-97
90
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.83
67.54
196.87
24.12
70.0
178.39
Standard deviation
2.29
6.25
15.16
2.32
7.94
16.8
C V (%)
8.87
9.25
7.7
9.62
11.34
9.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-12 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-12 in samples. No significant cross-reactivity or interference between Mouse IL-12 and analogues was observed.
