The Mouse IL-15 CLIA Kit is specifically designed for the precise measurement of interleukin-15 (IL-15) levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.IL-15 is a key cytokine involved in immune function, cell proliferation, and inflammation. It plays a critical role in the regulation of immune responses and has been implicated in various diseases, including autoimmune disorders and cancer.
Understanding the levels of IL-15 can provide valuable insights into the immune system and help develop targeted therapies for these conditions.With the Mouse IL-15 CLIA Kit, researchers can confidently study the role of IL-15 in immune responses, inflammation, and disease pathology. The high-quality components and user-friendly protocols make this kit an essential tool for investigating the complex interplay of cytokines in the immune system.
Product Name:
Mouse IL-15 CLIA Kit
SKU:
MOES00409
Target:
Mouse IL-15
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-15. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-15, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-15. You can calculate the concentration of Mouse IL-15 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
95-111
91-103
Average (%)
94
103
98
1:4
Range (%)
86-100
93-105
88-104
Average (%)
92
99
95
1:8
Range (%)
94-110
89-100
90-101
Average (%)
101
95
96
1:16
Range (%)
86-100
102-118
84-99
Average (%)
93
109
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
91-108
99
Cell culture media (n=5)
97-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.08
51.8
182.84
25.81
48.34
196.4
Standard deviation
2.9
4.08
19.18
2.97
3.88
19.38
C V (%)
11.12
7.88
10.49
11.51
8.03
9.87
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-15 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-15 in samples. No significant cross-reactivity or interference between Mouse IL-15 and analogues was observed.
