The Mouse IL-16 CLIA Kit is a highly sensitive and specific assay designed for the precise detection of interleukin-16 levels in mouse serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an excellent tool for researchers in various fields.Interleukin-16 is an important cytokine that plays a crucial role in immune responses, inflammation, and autoimmune diseases.
By measuring IL-16 levels, researchers can gain valuable insights into the immune system and its role in various diseases and conditions.With its high sensitivity and specificity, the Mouse IL-16 CLIA Kit offers researchers a reliable and efficient method for studying IL-16 and its implications in different research areas. Order this kit today from AssayGenie and take your research to the next level.
Product Name:
Mouse IL-16 CLIA Kit
SKU:
MOES00410
Target:
Mouse IL-16
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-16. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-16 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-16, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-16. You can calculate the concentration of Mouse IL-16 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
97-110
95-111
Average (%)
93
104
101
1:4
Range (%)
94-107
89-103
88-101
Average (%)
100
94
95
1:8
Range (%)
99-114
90-104
94-108
Average (%)
108
95
102
1:16
Range (%)
93-111
98-110
100-116
Average (%)
101
104
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
88-102
96
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.97
60.33
188.81
26.79
55.0
190.85
Standard deviation
2.49
6.9
16.12
3.14
5.69
19.47
C V (%)
9.23
11.44
8.54
11.72
10.35
10.2
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-16 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-16 in samples. No significant cross-reactivity or interference between Mouse IL-16 and analogues was observed.
