The Mouse IL-17 CLIA Kit is specifically designed for the quantitative detection of IL-17 levels in mouse serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing accurate and reliable results for a variety of research applications.IL-17 is a key cytokine involved in inflammatory responses and immune regulation. Elevated levels of IL-17 have been linked to autoimmune diseases, such as rheumatoid arthritis and multiple sclerosis.
By measuring IL-17 levels, researchers can gain valuable insights into the immune response and potential therapeutic targets for these conditions.The Mouse IL-17 CLIA Kit from AssayGenie offers a convenient and efficient solution for studying the role of IL-17 in mouse models. With its high performance and ease of use, this kit is a valuable tool for advancing immunology research and drug development efforts.
Product Name:
Mouse IL-17 CLIA Kit
SKU:
MOES00044
Target:
Mouse IL-17
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-17. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-17, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-17. You can calculate the concentration of Mouse IL-17 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
87-99
95-109
Average (%)
100
92
100
1:4
Range (%)
102-117
94-104
87-100
Average (%)
109
99
93
1:8
Range (%)
86-98
88-100
90-104
Average (%)
93
95
96
1:16
Range (%)
90-104
98-113
89-102
Average (%)
95
105
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
96
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
94-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.36
49.5
207.78
27.89
46.02
220.45
Standard deviation
3.14
5.37
23.6
2.52
3.95
21.12
C V (%)
11.48
10.85
11.36
9.04
8.58
9.58
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-17 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-17 in samples. No significant cross-reactivity or interference between Mouse IL-17 and analogues was observed.
