The Mouse IL18BP CLIA Kit is specifically designed for the quantitative measurement of Interleukin 18 Binding Protein (IL18BP) levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.IL18BP is a key regulator of the immune response, acting as a modulator of Interleukin 18 (IL-18) activity. It plays a critical role in inflammatory processes and immune system modulation, making it a valuable biomarker for studying immune-mediated diseases, autoimmune disorders, and inflammatory conditions.
With the Mouse IL18BP CLIA Kit, researchers can gain valuable insights into the role of IL18BP in various physiological and pathological processes, facilitating the development of novel therapeutic strategies and potential targeted treatments. Trust in the reliability and precision of this kit to advance your research in immunology and inflammation.
Product Name:
Mouse IL-18BP CLIA Kit
SKU:
MOES00419
Target:
Mouse IL-18BP
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL18BP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL18BP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL18BP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL18BP. You can calculate the concentration of Mouse IL18BP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
87-102
89-103
Average (%)
92
94
96
1:4
Range (%)
102-117
97-115
87-102
Average (%)
108
105
93
1:8
Range (%)
95-107
97-113
94-107
Average (%)
101
103
99
1:16
Range (%)
90-104
101-118
102-113
Average (%)
96
109
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
101
EDTA plasma (n=5)
95-112
103
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.5
259.24
922.69
100.8
273.54
836.88
Standard deviation
10.21
18.85
60.34
12.29
26.64
92.14
C V (%)
9.59
7.27
6.54
12.19
9.74
11.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL18BP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL18BP in samples. No significant cross-reactivity or interference between Mouse IL18BP and analogues was observed.
