The Mouse IL-1ra CLIA Kit is specifically designed for the sensitive detection of interleukin-1 receptor antagonist (IL-1ra) levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.IL-1ra is an important cytokine that functions as a natural antagonist to the pro-inflammatory effects of interleukin-1 (IL-1). It plays a critical role in regulating the immune response and inflammatory processes, making it a valuable biomarker for studying various diseases such as autoimmune disorders, inflammatory conditions, and infections.
Overall, the Mouse IL-1ra CLIA Kit provides researchers with a reliable tool for measuring IL-1ra levels in mouse samples, allowing for a better understanding of the role of this cytokine in disease pathogenesis and the development of potential therapeutic interventions.
Product Name:
Mouse IL-1ra CLIA Kit
SKU:
MOES00035
Target:
Mouse IL-1ra
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1ra. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1ra and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1ra, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-1ra. You can calculate the concentration of Mouse IL-1ra in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
88-99
100-116
Average (%)
99
93
107
1:4
Range (%)
86-101
94-109
84-99
Average (%)
93
101
90
1:8
Range (%)
101-115
96-108
92-105
Average (%)
108
102
98
1:16
Range (%)
99-113
95-110
101-113
Average (%)
105
103
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-112
106
EDTA plasma (n=5)
95-111
103
Cell culture media (n=5)
98-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.61
118.85
395.82
39.5
128.09
389.86
Standard deviation
4.09
9.48
26.01
4.0
9.48
28.34
C V (%)
11.17
7.98
6.57
10.13
7.4
7.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-1ra concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-1ra in samples. No significant cross-reactivity or interference between Mouse IL-1ra and analogues was observed.
