The Mouse IL-21 CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of IL-21 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it perfect for a variety of research applications.IL-21 is a key cytokine involved in immune responses, functioning in the regulation of T and B cell activation and differentiation. It plays a crucial role in autoimmune diseases, allergies, and inflammatory conditions, making it a valuable marker for studying these diseases and developing new therapeutic strategies.
With its exceptional performance and accuracy, the Mouse IL-21 CLIA Kit from Assay Genie is an indispensable tool for researchers looking to advance their understanding of immune responses and inflammatory conditions in mouse models.
Product Name:
Mouse IL-21 CLIA Kit
SKU:
MOES00602
Target:
Mouse IL-21
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-21. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-21, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-21. You can calculate the concentration of Mouse IL-21 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
94-108
96-108
Average (%)
94
99
102
1:4
Range (%)
91-106
102-118
100-116
Average (%)
96
109
106
1:8
Range (%)
94-105
102-115
97-109
Average (%)
100
108
104
1:16
Range (%)
96-111
91-106
94-108
Average (%)
103
99
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
93
EDTA plasma (n=5)
85-95
90
Cell culture media (n=5)
100-118
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.59
125.37
361.74
42.0
113.28
333.34
Standard deviation
5.1
13.88
26.01
4.63
13.53
38.23
C V (%)
12.56
11.07
7.19
11.02
11.94
11.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-21 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-21 in samples. No significant cross-reactivity or interference between Mouse IL-21 and analogues was observed.
