The Mouse IL-24 (Interleukin 24) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of IL-24 levels in mouse serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an excellent tool for various research applications.IL-24 is a cytokine involved in immune regulation and inflammation, with potential roles in cancer therapy and autoimmune diseases.
By measuring IL-24 levels, researchers can gain valuable insights into the immune response and disease progression, ultimately aiding in the development of targeted therapies.With its high performance and ease of use, the Mouse IL-24 ELISA Kit from Assay Genie is a valuable asset for researchers studying cytokine signaling pathways and investigating the role of IL-24 in various physiological and pathological processes.
Product Name:
Mouse IL-24 ELISA Kit
SKU:
MOES00664
Target:
Mouse IL-24
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-24. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-24 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-24, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-24. You can calculate the concentration of Mouse IL-24 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
85-96
83-98
Average (%)
105
90
90
1:4
Range (%)
99-111
81-94
98-112
Average (%)
105
87
105
1:8
Range (%)
98-116
85-101
95-107
Average (%)
106
92
101
1:16
Range (%)
100-119
83-97
92-107
Average (%)
108
90
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
98
EDTA plasma (n=5)
93-105
98
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.6
182.5
915.3
106.4
176.0
837.7
Standard deviation
6.7
10.0
29.3
6.0
8.6
37.7
C V (%)
6.86
5.48
3.2
5.64
4.89
4.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-24 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-24 in samples. No significant cross-reactivity or interference between Mouse IL-24 and analogues was observed.
