The Mouse IL2R CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of IL2R levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and consistent results, making it ideal for a variety of research applications.IL2R, also known as interleukin-2 receptor, plays a critical role in the immune response by regulating T cell activation and proliferation. Dysregulation of IL2R has been implicated in various autoimmune and inflammatory diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With its easy-to-use format and high performance characteristics, the Mouse IL2R CLIA Kit is a valuable tool for researchers in immunology, oncology, and other fields interested in exploring the role of IL2R in disease pathology and treatment development.
Product Name:
Mouse IL-2R CLIA Kit
SKU:
MOES00415
Target:
Mouse IL-2R
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-2R. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-2R and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-2R, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-2R. You can calculate the concentration of Mouse IL-2R in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
92-105
87-101
Average (%)
96
99
93
1:4
Range (%)
95-109
89-100
85-97
Average (%)
103
94
90
1:8
Range (%)
88-101
95-110
100-115
Average (%)
94
102
108
1:16
Range (%)
87-100
87-98
92-106
Average (%)
92
93
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
101
EDTA plasma (n=5)
101-113
107
Cell culture media (n=5)
89-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.14
49.09
239.54
21.95
46.5
241.4
Standard deviation
2.75
4.97
15.55
2.42
5.0
15.55
C V (%)
11.88
10.12
6.49
11.03
10.75
6.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-2R concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-2R in samples. No significant cross-reactivity or interference between Mouse IL-2R and analogues was observed.
