The Mouse IL-3 ELISA Kit is a powerful tool for detecting and quantifying Interleukin-3 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for various research applications.Interleukin-3 is a vital cytokine that regulates the growth and differentiation of a variety of immune cells, including mast cells, eosinophils, and basophils. It is also involved in inflammation and allergic responses, making it a key target for studying immune-related diseases and developing new therapeutic strategies.
Whether investigating immune system function, studying inflammatory diseases, or exploring potential treatments, the Mouse IL-3 ELISA Kit is an essential tool for researchers seeking to better understand the role of Interleukin-3 in mouse models. Order yours today and take your research to the next level.
Product Name:
Mouse IL-3 ELISA Kit
SKU:
MOES01216
Target:
Mouse IL-3
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-3. You can calculate the concentration of Mouse IL-3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
89-105
92-107
Average (%)
97
96
98
1:4
Range (%)
92-107
85-97
84-97
Average (%)
99
90
90
1:8
Range (%)
87-98
83-98
85-100
Average (%)
92
90
92
1:16
Range (%)
90-101
82-93
80-94
Average (%)
95
89
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
93-107
100
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.4
142.23
353.93
53.65
137.47
331.67
Standard deviation
2.85
7.14
18.4
2.8
5.99
10.02
C V (%)
5.24
5.02
5.2
5.22
4.36
3.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-3 in samples. No significant cross-reactivity or interference between Mouse IL-3 and analogues was observed.
