The Mouse IL35 CLIA Kit is a powerful tool for researchers looking to accurately measure levels of IL35 in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, delivering dependable and consistent results for a variety of experimental purposes.IL35 is a key cytokine involved in modulating immune responses, particularly in regulating immune tolerance and suppressing inflammatory reactions. Dysregulation of IL35 has been implicated in various autoimmune diseases and chronic inflammation conditions, highlighting its importance as a biomarker for studying these disorders and exploring potential therapeutic interventions.
With the Mouse IL35 CLIA Kit, researchers can confidently measure IL35 levels in biological samples, advancing their understanding of immune regulation and potentially identifying new targets for therapeutic intervention.
Product Name:
Mouse IL-35 CLIA Kit
SKU:
MOES00414
Target:
Mouse IL-35
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-35. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-35 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-35, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-35. You can calculate the concentration of Mouse IL-35 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
98-114
96-110
Average (%)
92
105
104
1:4
Range (%)
90-104
99-110
92-106
Average (%)
97
104
99
1:8
Range (%)
101-114
98-110
93-108
Average (%)
107
103
100
1:16
Range (%)
95-108
86-101
95-106
Average (%)
101
92
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
99-111
106
Cell culture media (n=5)
96-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.71
48.44
178.35
28.65
52.61
186.77
Standard deviation
3.14
4.7
17.44
3.46
4.45
14.87
C V (%)
11.76
9.7
9.78
12.08
8.46
7.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-35 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-35 in samples. No significant cross-reactivity or interference between Mouse IL-35 and analogues was observed.
