The Mouse IL-5 CLIA Kit (MOES00403) from Assay Genie is a cutting-edge assay tool designed for the precise measurement of Interleukin-5 (IL-5) levels in mouse samples including serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications. IL-5 is a critical cytokine that plays a vital role in regulating immune responses, particularly in the activation and differentiation of eosinophils.
Dysregulation of IL-5 has been implicated in various inflammatory conditions such as asthma and allergies, making it a valuable marker for studying these diseases and identifying potential treatments. With the Mouse IL-5 CLIA Kit (MOES00403), researchers can easily quantify IL-5 levels in mouse models, providing valuable insights into immune function and inflammatory processes. Trust Assay Genie for reliable and high-quality assay kits for your research needs.
Product Name:
Mouse IL-5 CLIA Kit
SKU:
MOES00403
Target:
Mouse IL-5
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-5. You can calculate the concentration of Mouse IL-5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
86-97
87-99
Average (%)
96
91
92
1:4
Range (%)
92-107
98-115
99-116
Average (%)
97
105
107
1:8
Range (%)
98-113
91-105
89-105
Average (%)
106
98
97
1:16
Range (%)
93-106
96-108
91-103
Average (%)
99
101
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
96
EDTA plasma (n=5)
95-110
101
Cell culture media (n=5)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.79
41.75
223.89
27.68
41.66
233.34
Standard deviation
2.87
4.73
22.39
2.86
3.19
17.73
C V (%)
11.13
11.33
10.0
10.33
7.66
7.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-5 in samples. No significant cross-reactivity or interference between Mouse IL-5 and analogues was observed.
