The Mouse IL-7 CLIA Kit is specifically designed for the quantification of interleukin-7 (IL-7) levels in mouse serum, plasma, and tissue homogenates. This kit utilizes a Chemiluminescent Immunoassay (CLIA) technology that offers high sensitivity and accuracy, providing reliable and reproducible results for research applications.IL-7 is a vital cytokine that plays a crucial role in the development and homeostasis of immune cells, particularly T cells. Dysregulation of IL-7 has been implicated in various immune-related diseases, making it a valuable biomarker for studying immune disorders and evaluating potential therapies.
This Mouse IL-7 CLIA Kit from Assay Genie is a valuable tool for researchers looking to investigate the role of IL-7 in immune system function and disease pathogenesis. With its easy-to-use format and superior performance, this kit is an essential addition to any laboratory studying immune responses in mouse models.
Product Name:
Mouse IL-7 CLIA Kit
SKU:
MOES00404
Target:
Mouse IL-7
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-7. You can calculate the concentration of Mouse IL-7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-119
88-100
87-101
Average (%)
108
93
94
1:4
Range (%)
91-106
94-109
102-116
Average (%)
98
101
109
1:8
Range (%)
102-118
95-105
92-107
Average (%)
109
100
99
1:16
Range (%)
99-115
99-113
87-99
Average (%)
106
105
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
104
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
92-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.65
52.79
215.05
24.86
51.11
213.81
Standard deviation
2.54
5.24
23.18
2.48
4.34
19.84
C V (%)
9.53
9.93
10.78
9.98
8.49
9.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-7 in samples. No significant cross-reactivity or interference between Mouse IL-7 and analogues was observed.
