The Mouse IL9 CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of mouse interleukin-9 (IL-9) levels in biological samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.IL-9 is a cytokine that plays a crucial role in regulating immune responses, particularly in allergic diseases and inflammatory conditions. It is involved in the differentiation and activation of various immune cells, making it an important marker for studying immune system disorders and developing potential therapies.
With its precision and efficiency, the Mouse IL9 CLIA Kit is a valuable tool for researchers looking to investigate the role of IL-9 in immune regulation and disease pathology. Its high performance and ease of use make it essential for any laboratory working in the field of immunology and inflammation research.
Product Name:
Mouse IL-9 CLIA Kit
SKU:
MOES00405
Target:
Mouse IL-9
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-9. You can calculate the concentration of Mouse IL-9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
86-99
100-114
Average (%)
99
93
108
1:4
Range (%)
103-120
88-98
94-107
Average (%)
109
93
100
1:8
Range (%)
100-113
88-102
91-106
Average (%)
107
93
98
1:16
Range (%)
91-104
94-108
89-103
Average (%)
98
100
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
92
EDTA plasma (n=5)
96-110
104
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.1
75.08
213.25
24.25
82.32
231.17
Standard deviation
2.46
5.75
18.66
2.4
9.66
24.74
C V (%)
10.65
7.66
8.75
9.9
11.73
10.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-9 in samples. No significant cross-reactivity or interference between Mouse IL-9 and analogues was observed.
