SynonymsINDOL1,Indoleamine-Pyrrole 2,3 Dioxygenase-Like 1,Indoleamine 2,3-dioxygenase-like protein 1,Indoleamine-pyrrole 2,3-dioxygenase-like protein 1
ReactivityMouse
Sample TypeSerum, Plasma, Tissue Homogenates And Other Biological Fluids
SKU:
MODL00657
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8R0V5
Sensitivity:
0.32ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
INDOL1,Indoleamine-Pyrrole 2,3 Dioxygenase-Like 1,Indoleamine 2,3-dioxygenase-like protein 1,Indoleamine-pyrrole 2,3-dioxygenase-like protein 1
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
The Mouse Indoleamine 2,3-Dioxygenase 2 (IDO2) ELISA Kit is a powerful tool for the accurate measurement of IDO2 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it suitable for a variety of research applications.IDO2 is an enzyme that plays a critical role in the regulation of immune responses and inflammation. It is involved in the metabolism of tryptophan and has been implicated in various diseases, including autoimmune disorders, cancer, and infectious diseases.
By measuring IDO2 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and identify potential therapeutic targets.Overall, the Mouse IDO2 ELISA Kit offers researchers a valuable tool for studying the role of IDO2 in immune regulation and disease pathogenesis, ultimately contributing to the development of novel therapeutic approaches.
INDOL1, Indoleamine-Pyrrole 2,3 Dioxygenase-Like 1, Indoleamine 2,3-dioxygenase-like protein 1, Indoleamine-pyrrole 2,3-dioxygenase-like protein 1
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
