The Mouse INHA (Inhibin A) CLIA Kit is specifically designed for the quantitative detection of Inhibin A levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it an essential tool for research in various areas.Inhibin A is a key protein that plays a crucial role in reproductive health and hormone regulation. It is involved in the inhibition of follicle-stimulating hormone (FSH) secretion, which is essential for the regulation of ovarian function and follicular development.
The measurement of Inhibin A levels is important for understanding reproductive disorders, infertility, and gonadal function in animals.This Mouse INHA (Inhibin A) CLIA Kit is an invaluable resource for researchers studying reproductive biology, endocrinology, and animal physiology. Its reliability and precision make it an indispensable tool for conducting experiments and investigations in the field of reproductive science.
Product Name:
Mouse INHA (Inhibin A) CLIA Kit
SKU:
MOES00382
Target:
Mouse INHA (Inhibin A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse INHA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INHA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INHA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse INHA. You can calculate the concentration of Mouse INHA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-111
85-96
95-109
Average (%)
101
90
101
1:4
Range (%)
85-97
101-115
99-112
Average (%)
92
108
105
1:8
Range (%)
88-101
91-102
104-117
Average (%)
93
96
110
1:16
Range (%)
91-103
98-113
99-112
Average (%)
97
106
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
104
EDTA plasma (n=5)
85-101
92
Cell culture media (n=5)
96-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.78
51.26
224.72
28.89
51.01
241.35
Standard deviation
3.15
4.88
18.09
3.16
4.89
26.04
C V (%)
11.76
9.52
8.05
10.94
9.59
10.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse INHA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse INHA in samples. No significant cross-reactivity or interference between Mouse INHA and analogues was observed.
