The Mouse INSL5 (Insulin-Like Protein 5) CLIA Kit is a highly sensitive and specific assay designed to accurately measure levels of INSL5 in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an excellent tool for various research applications.INSL5 is a key protein that plays a role in metabolic regulation, particularly in the gastrointestinal tract.
Its functions include promoting appetite and regulating glucose metabolism, making it a crucial biomarker for studying metabolic disorders and potential therapeutic interventions. This kit is essential for researchers interested in understanding the mechanisms behind metabolic regulation and exploring new treatment options for related conditions.
Product Name:
Mouse INSL5 (Insulin Like Protein 5) CLIA Kit
SKU:
MOES00386
Target:
Mouse INSL5 (Insulin Like Protein 5)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse INSL5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INSL5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INSL5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse INSL5. You can calculate the concentration of Mouse INSL5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-116
87-99
84-97
Average (%)
106
93
91
1:4
Range (%)
96-111
91-107
89-104
Average (%)
102
98
96
1:8
Range (%)
95-112
85-95
92-105
Average (%)
103
90
98
1:16
Range (%)
84-100
93-106
99-115
Average (%)
91
101
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
105
EDTA plasma (n=5)
99-114
105
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.29
72.34
194.74
25.87
66.29
204.05
Standard deviation
3.51
6.88
18.68
2.77
7.27
20.63
C V (%)
12.86
9.51
9.59
10.71
10.97
10.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse INSL5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse INSL5 in samples. No significant cross-reactivity or interference between Mouse INSL5 and analogues was observed.
