Mouse Insulin Like Growth Factor 2 (IGF2) ELISA Kit
The Mouse Insulin-like Growth Factor 2 (IGF2) ELISA Kit is a powerful tool for accurately measuring levels of IGF2 in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers precise and reliable results, making it the perfect choice for a variety of research applications.IGF2 is a key growth factor that plays a critical role in regulating cell growth, differentiation, and development. It is involved in various physiological processes, including embryonic development, tissue regeneration, and metabolism.
Dysregulation of IGF2 has been linked to numerous diseases, such as cancer, diabetes, and growth disorders, highlighting its importance as a biomarker for studying these conditions and exploring potential therapeutic interventions.Get your hands on the Mouse IGF2 ELISA Kit today and accelerate your research in the field of growth factors and developmental biology!
Product Name:
Mouse Insulin Like Growth Factor 2 (IGF2) ELISA Kit
Product Code:
MODL00662
Size:
96 Assays
Target:
IGF2
Synonyms:
INSIGF, IGF-II, Somatomedin A, Preptin, Insulin-like growth factor II Ala-25 Del
Detection Method:
Sandwich
Range:
93.75-6000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
