Mouse Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit
The Mouse Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit is specifically designed for the accurate detection and quantification of IGFBP3 levels in mouse serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring dependable and reproducible results for various research applications.IGFBP3 is a key binding protein involved in regulating the bioavailability and activity of insulin-like growth factors (IGFs). It plays a vital role in cell growth, differentiation, and apoptosis, making it a critical marker for studying mechanisms related to growth and development.
Elevated levels of IGFBP3 have been associated with certain diseases such as cancer, diabetes, and growth disorders, highlighting its importance in understanding these conditions and potentially developing targeted interventions.Overall, the Mouse IGFBP3 ELISA Kit provides researchers with a powerful tool to investigate the role of IGFBP3 in various physiological and pathological processes, offering valuable insights for advancing scientific knowledge and therapeutic strategies.
Product Name:
Mouse Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit
Product Code:
MODL00665
Size:
96 Assays
Target:
IGFBP3
Synonyms:
BP53, IBP3, Growth Hormone-Dependent Binding Protein, Acid Stable Subunit Of The 140 K IGF Complex
Detection Method:
Sandwich
Range:
4.687-300ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
