The Mouse Integrin Alpha 4 (ITGA4) ELISA Kit is a reliable tool for the sensitive detection of ITGA4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity and accuracy, ensuring consistent and dependable results for various research purposes.ITGA4 is a key integrin protein that plays a crucial role in cell adhesion and migration, making it essential for processes like immune response and tissue regeneration.
Dysregulation of ITGA4 has been linked to various diseases, including inflammatory disorders and cancer, highlighting its significance as a biomarker for disease research and therapeutic development.Overall, the Mouse Integrin Alpha 4 ELISA Kit is a valuable resource for researchers looking to study the role of ITGA4 in health and disease, providing reliable data for advancing scientific understanding and potential clinical applications.
Product Name:
Mouse Integrin Alpha 4 (ITGa4) ELISA Kit
Product Code:
MODL00745
Size:
96 Assays
Target:
ITGa4
Synonyms:
CD49d, CD49-D, ITG-A4, IA4, Alpha 4 Subunit Of VLA-4 Receptor, CD49 antigen-like family member D, Integrin alpha-IV
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
