The Mouse ITLN1 (Intelectin-1/Omentin) CLIA Kit is specifically designed for the precise and accurate measurement of ITLN1 levels in mouse serum, plasma, and cell culture supernatants. This high-quality kit guarantees exceptional sensitivity and specificity, ensuring trustworthy and consistent results for various research purposes.ITLN1, also known as Intelectin-1 or Omentin, is a critical protein involved in immune response modulation and metabolic regulation. It plays a vital role in conditions such as obesity, insulin resistance, and inflammatory diseases, making it a valuable biomarker for investigating these diseases and exploring potential therapeutic interventions.
Don't miss out on the opportunity to advance your research with the Mouse ITLN1 CLIA Kit, a reliable tool for studying the complex mechanisms of ITLN1 in mouse models. Visit the link for more information: www.assaygenie.com/mouse-itln1-intelectin-1-omentin-clia-kit-moes00460/
Product Name:
Mouse ITLN1 (Intelectin 1/Omentin) CLIA Kit
SKU:
MOES00460
Target:
Mouse ITLN1 (Intelectin 1/Omentin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ITLN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ITLN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ITLN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ITLN1. You can calculate the concentration of Mouse ITLN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
95-110
98-112
Average (%)
103
101
106
1:4
Range (%)
90-105
101-115
100-112
Average (%)
97
109
106
1:8
Range (%)
103-119
91-104
91-104
Average (%)
109
97
96
1:16
Range (%)
88-102
97-113
93-107
Average (%)
94
105
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
98
EDTA plasma (n=5)
96-110
103
Cell culture media (n=5)
91-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
196.56
628.12
1956.0
188.16
690.47
1810.14
Standard deviation
25.1
47.17
173.69
22.77
66.01
196.22
C V (%)
12.77
7.51
8.88
12.1
9.56
10.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ITLN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ITLN1 in samples. No significant cross-reactivity or interference between Mouse ITLN1 and analogues was observed.
