The Mouse Ki-67 Protein (Ki67P) ELISA Kit is specifically designed for the accurate measurement of Ki-67 protein levels in mouse samples including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and consistent results, making it an excellent choice for various research applications. The Ki-67 protein is a well-known marker for cell proliferation and is commonly used in studies related to cancer, cell cycle progression, and tumor growth. By accurately quantifying levels of Ki-67 protein, researchers can gain valuable insights into cellular activity and disease progression, paving the way for the development of innovative therapies and interventions.
With easy-to-follow instructions and all necessary components included, the Mouse Ki-67 Protein (Ki67P) ELISA Kit offers a convenient and efficient solution for scientists looking to study Ki-67 protein expression in mouse models. Choose this kit for reliable and reproducible results in your research endeavors.
Product Name:
Mouse Ki-67 Protein (Ki67P) ELISA Kit
Product Code:
MODL00761
Size:
96 Assays
Target:
Ki67P
Synonyms:
MKI67, KIA, Ki-67, Antigen Identified By Monoclonal Antibody Ki-67
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-100
95
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
