Mouse LAT (Linker for activation of T cell) CLIA Kit
The Mouse LAT linker for Activation of T cell CLIA Kit is specifically designed for the detection of LAT (Linker for Activation of T cells) levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing accurate and reliable results for your research needs.LAT is a key protein involved in T cell activation and signaling, playing a critical role in the immune response. By measuring LAT levels, researchers can gain valuable insights into the functioning of T cells and their role in immune regulation, making this kit essential for studies in immunology, oncology, and autoimmune diseases.
With its user-friendly protocol and robust performance, the Mouse LAT linker for Activation of T cell CLIA Kit is an indispensable tool for researchers looking to deepen their understanding of T cell biology and develop novel therapeutic strategies.
Product Name:
Mouse LAT (Linker for activation of T cell) CLIA Kit
SKU:
MOES00425
Target:
Mouse LAT (Linker for activation of T cell)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LAT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LAT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse LAT. You can calculate the concentration of Mouse LAT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
94-107
104-116
Average (%)
98
100
110
1:4
Range (%)
102-117
99-113
90-101
Average (%)
109
107
95
1:8
Range (%)
98-112
100-118
99-115
Average (%)
103
108
107
1:16
Range (%)
85-97
84-100
99-112
Average (%)
91
91
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
99-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.31
227.83
916.73
104.85
219.9
946.12
Standard deviation
8.42
16.15
93.87
10.15
24.98
61.21
C V (%)
8.15
7.09
10.24
9.68
11.36
6.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse LAT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LAT in samples. No significant cross-reactivity or interference between Mouse LAT and analogues was observed.
